Oocytes Population and Development Competence of Bali Cattle Embryo In Vitro with Different Ovarian Reproductive Statuses
نویسندگان
چکیده
The present study aims to determine the potential of Bali cattle ovaries as sources oocytes for in vitro embryo production based on different ovarian reproductive statuses. were grouped into 4 categories: with no corpus luteum and dominant follicles (CL-DF-), those (CL+DF-), without but (CL-DF+), follicle (CL+DF+). collected via slicing technique grades (a, b, c, d). oocyte’s maturation was performed using tissue culture medium 199 basic media. A drop sample (10–15 oocytes/drop) covered mineral oil then placed a 5% CO2 incubator at temperature 38.5 ℃ 24 h. Then, samples fertilized 80 μL fertilization final spermatozoa concentration 1.5×106 spermatozoa/mL. After 5–6 h fertilization, washed four times Charles Rosenkrans 1aa (CR1aa) medium. cultured CR1aa base results showed significant difference (p>0.05) groups oocyte population from one pair ovary well number that suitable maturation. However, group CL-DF+ (p<0.05) rate nuclear (80.00±12.84), (80.00±4.72), ability development (60.19±22.45) when compared CL-DF-, CL+DF-, CL+DF+. This determines pairs quantity are fit is not influenced by status ovaries. level maturation, higher follicles.
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ژورنال
عنوان ژورنال: Tropical Animal Science Journal
سال: 2022
ISSN: ['2615-787X', '2615-790X']
DOI: https://doi.org/10.5398/tasj.2022.45.4.389